Analysis of Mammary Tissue for Diagnostic Modeling

Market Overview:

This fine needle biopsy method is used to evaluate mammary tissue collected repetitively over time from the same animal during development, allowing researchers to predict productivity, the animal’s quality, health, and chance of disease. The U.S. meat and livestock market is a growing market, forecast to have a value of $203.5 billion by 2019. Currently, the market lacks a model to provide predictive values and better understand animal disease. This poses problems when it comes to understanding cell signaling events in mammals, assessing beef quality, and making genetic decisions regarding animal productivity. Clemson University researchers have developed a non-invasive biopsy procedure that allows for mammary tissue to be analyzed from the same animal over time, therefore, decreasing variation and providing a way to better predict the animal’s comprehensive health. This approach is safe, cost effective, time sensitive and could generate a predictive value for animal productivity and well-being before the animal reaches maturity. 

 

Application                                                                                        Stage of Development

Animal health diagnostics; mammary development models              Animal study validation

 

Advantages

•  Uses a non-invasive, fine needle biopsy to safely collect tissue samples from the same animal over time,

   eliminating variability that results from using different animals

•  Generates a predictive value for an animal’s productivity before it reaches reproductive age, allowing better

   management decisions to be made

•  Provides a way to analyze the role of development signaling pathways in the progression of disease in

   animals, leading to a better understanding of pathophysiology

 

Technical Summary

This method uses a sterile, fine-needle biopsy device to collect mammary tissue samples once a week during the course of early development. The samples are either paraffin embedded and stained for use in fluorescence microscopy or RNA isolation is performed to evaluate changes in cell signaling over time. This data is then used as a genomic indicator of animal productivity. Recently sequenced transcriptomic data has shown significantly up and down-regulated genes beginning in one-week old animals. The predictive genetic value provided from gene expression will vastly increase the degree of sensitivity at the molecular level of evaluation. Lastly, a comprehensive prediction can be made regarding the animal’s health and productivity during its life post-development. This method is repeatable and reproducible, low-cost, and has the potential to allow early prediction of animal health unlike traditional methods of post-mortem analysis.

 

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Inventors:                          Heather Dunn, Kathryn A Elliott, Thomas Scott, Matthew Burns

 

Patent Type:                      Provisional

 

Serial Number:                  62/256,416

 

CURF Reference:              2015-056

 

Patent Information:
Category(s):
Agriculture
Biotechnology
For Information, Contact:
Chris Gesswein
Director of Licensing
Clemson University Research Foundation
agesswe@clemson.edu
Inventors:
Heather Dunn
Kathryn Elliott
Thomas Scott
Matthew Burns
Keywords:
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